The Problem

Today, one of the most promising approaches in regenerative medicine for treating tissue defects is tissue engineering. This method involves using matrices (scaffolds) to create a framework for damaged organs or organ components, which are then populated with biologically active substances.

The goal of the proposed project is to develop an innovative product for tissue regeneration that utilises a bacterial cellulose scaffold enriched with autologous extracellular growth factors. This combination aims to enhance the regenerative process, promoting effective healing and tissue restoration.

Our company has deposited bacterial strains responsible for the production of bacterial cellulose. The Medusomyces gisevii culture was deposited and assigned the registration number Sa-28. Five strains of microorganisms were isolated from this culture, including one bacterial culture and four yeast. All five cultures were deposited and assigned registration numbers:

• Yeast culture Brettanomyces bruxellensis ink-01-1 (Y-5060);
• Yeast culture Brettanomyces bruxellensis ink-01-2 (Y-5061);
• Yeast culture Brettanomyces bruxellensis ink-01-3 (Y-5062);
• Yeast culture Pichia manshurica ink-01-4 (Y-5063);
• Bacterial culture Komagataeibacter xylinus ink-01-5 (B-14280).

The potential for utilizing bacterial cellulose (BC) and scaffolds derived from it in pharmaceuticals and medicine highlights the importance of purity and the absence of bacterial endotoxins as critical characteristics. Endotoxins are components found within certain bacteria that are released only when the bacterial cells are lysed. Ensuring that BC and its scaffolds are free from these contaminants is essential for their safety and efficacy in medical applications.

All produced scaffold samples demonstrate high efficiency. The scaffold made from pure bacterial cellulose (BC) (No. 1) has a moisture capacity of 2714%. Notably, the inclusion of additional components further enhances this moisture capacity. Analysis shows that the addition of mixed peptides, irrespective of whether a formative agent is present, results in a moisture capacity exceeding 5000%.

Furthermore, all scaffold samples exhibit a high porosity index, ranging from 95% to 98.7%. These exceptional moisture retention and porosity indices indicate the superior quality of all the obtained scaffold samples.

When studying bacterial cellulose on a chicken embryo model, the high level of biocompatibility of the selected scaffold variants was confirmed.

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